α-2-6-sialyltransferase

EV-3002

Recombinant bacterial α-2,6-sialyltransferase (α2,6SiaT)
Synonyms: β-Galactoside α-2,6-sialyltransferase
CAS No.: n/a
Molecular weight: 45 kDa

Quantity

€ 580.00 EUR

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Product Specs

Technical product information

Description

This recombinant bacterial α-2,6-sialyltransferase (α2,6SiaT) is expressed in E. coli BL21(DE3) and is designed for sialylation of glycoproteins, glycolipids and free glycans including oligosaccharides. It catalyzes the transfer of sialic acid from CMP-Neu5Ac (or other CMP-Neu5Ac analogues) to terminal galactose residues in an α-2,6 linkage. Compared to other known α2,6SiaTs, this enzyme has suppressed hydrolytic activities, resulting in enhanced synthesis efficiency.

Other identifiers

Unit definition (U): formation of 1 µmol 6'SL from 1 mM CMP-Neu5Ac and 1 mM lactose per minute at 37 °C, pH 8.0.

Appearance

Lyophilized powder (MOPS and NaCl)

Molecular Weight (MW)

45 kDa

Expression host

E. coli BL21(DE3)

Purity

> 90 % (SDS-PAGE)

Solubility

Storage

At -20 °C

Special instructions

For research use only. Not for use in diagnostic procedures.

His-tag

Yes

Reconstitution

Add 10–100 µL deionized water.

Toxicity & Safety

Enzymatic activity characterisation

• Donor substrate: CMP-Neu5Ac

• Acceptor substrate: Glycans with terminal galactose

• Reaction conditions: pH 6.5–8.0; 25–37 °C

• Transferase activity: α-2,6-sialyltransferase

• Hydrolytic activity: Trace

• Sialidase activity: Undetectable

Availability

Application Examples

• For the sialylation of a wide range of glycans with galactose terminal: G2F, G2, Lewis a, Lewis x, lactose, LNT, LNnT, etc.

• For the sialylation of glycoproteins: Rituximab (in combination with β1,4-GalT), Erythropoietin (EPO) for terminal sialylation, Fc-fusion proteins for enhanced serum half-life

• Glycan arrays for binding studies

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Product Datasheet / CoA / MSDS

Technical Product information

Sample CoA

MSDS

MSDS - animal free

Citations