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Until 31.12.2024: Save 30% on your first order with code: eversyn30

alpha-1,3-galactosyltransferase

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€490.00

Recombinant α-1,3-Galactosyltransferase (α1,3GalT) from bovine origin expressed in E. coli enables in vitro galactosylation of glycoproteins. The enzyme catalyzes the transfer of galactose from UDP-galactose to biantennary N-glycans with terminal galactose residues, forming α-1,3- linkages.

 

The enzyme is for research use only. Not for use in diagnostic procedures.

 

The product is available only upon request.

 

Pack sizes:

  • 50EqU: are suitable to catalyze the in vitro galactosylation of 1 mg glycoprotein
  • 500EqU: are suitable to catalyze the in vitro galactosylation of 10 mg glycoprotein

 

Equivalent Unit (EqU) definition: Is the amount of enzyme required to achieve complete conversion of 3 pmol of G2F to G2FG2 in 24 h at 25 °C.

Size

Quantity

Please contact us at info@eversyn.de if you are interested in this product.

Technical Specifications

Parameter Specification
Molecular weight 35 kDa
Expression host E. coli BL21(DE3)
Purity > 90 % (SDS-PAGE)
Purification tag His-tag
Form Lyophilized powder
(MOPS and NaCl)
Reconstitution Add 10–100 μL
deionized water
Store at -20 °C

 

Enzymatic activity characterisation

Donor substrate UDP-galactose
Acceptor substrate Glycans with terminal galactose
Reaction conditions pH 6.5–8.0; 25–37 °C
Transferase activity α-1,3-galactosyltransferase
Reaction conditions Recommended pH 6.6, 20°C

 

Application examples

  • Glycoengineering of glycoproteins
  • Glycan structure-function studies
  • Biopharmaceutical glycoform optimization
  • Development of glycan-based therapeutics
  • Enzymatic synthesis of glycan standards
  • Functional glycomics research

For larger quantities or custom synthesis

Get in touch at info@eversyn.de for more details.

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In-stock products are usually shipped within 2 days after order confirmation.

For larger amounts or higher purity / custom synthesis

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